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    Cervical Cancer Awareness - Key to Curb New Incidences sunishtha s yadav snps cancer incidences Cervical Cancer Awareness - Key to Curb New Incidences купить
    Cervical Cancer Awareness - Key to Curb New Incidences
    4929 Руб
      Cancer of the Oesophagus in Zambia sunishtha s yadav snps cancer incidences Cancer of the Oesophagus in Zambia купить
      Cancer of the Oesophagus in Zambia
      3549 Руб
        DNA biosensors sunishtha s yadav snps cancer incidences DNA biosensors купить
        DNA biosensors
        4929 Руб
          Cancer Incidence Risks and Patient Dose Due to Fluoroscopic Procedures sunishtha s yadav snps cancer incidences Cancer Incidence Risks and Patient Dose Due to Fluoroscopic Procedures купить
          Cancer Incidence Risks and Patient Dose Due to Fluoroscopic Procedures
          5790 Руб
            Early-life alcoholism and prostate cancer risk: what is the relation? sunishtha s yadav snps cancer incidences Early-life alcoholism and prostate cancer risk: what is the relation? купить
            Early-life alcoholism and prostate cancer risk: what is the relation?
            3549 Руб
              Computational Analysis of SNPs and Codons for cancerous genes sunishtha s yadav snps cancer incidences Computational Analysis of SNPs and Codons for cancerous genes купить
              Computational Analysis of SNPs and Codons for cancerous genes
              5790 Руб
                Dreambag Кресло-мешок dreambag comfort indigo,~(SNPS-WOH) sunishtha s yadav snps cancer incidences Dreambag Кресло-мешок dreambag comfort indigo,~(SNPS-WOH) купить
                Dreambag Кресло-мешок dreambag comfort indigo,~(SNPS-WOH)
                3889 Руб
                  Buffalo myogenic genes and their association with growth traits sunishtha s yadav snps cancer incidences Buffalo myogenic genes and their association with growth traits купить
                  Buffalo myogenic genes and their association with growth traits
                  4929 Руб
                    SNPs in yeast for the detection of genes and biodiversity sunishtha s yadav snps cancer incidences SNPs in yeast for the detection of genes and biodiversity купить
                    SNPs in yeast for the detection of genes and biodiversity
                    4839 Руб
                      NUTRITIONAL PHARMACOLOGY OF DIETARY PHYTOCHEMICALS sunishtha s yadav snps cancer incidences NUTRITIONAL PHARMACOLOGY OF DIETARY PHYTOCHEMICALS купить
                      NUTRITIONAL PHARMACOLOGY OF DIETARY PHYTOCHEMICALS
                      5790 Руб
                        Validation of deep sequencing data from Drosophila mauritiana sunishtha s yadav snps cancer incidences Validation of deep sequencing data from Drosophila mauritiana купить
                        Validation of deep sequencing data from Drosophila mauritiana
                        2359 Руб
                          Cancer Biology sunishtha s yadav snps cancer incidences Cancer Biology купить
                          Cancer Biology
                          4839 Руб
                            Детская футболка классическая унисекс Printio Totem cancer sunishtha s yadav snps cancer incidences Детская футболка классическая унисекс Printio Totem cancer купить
                            Детская футболка классическая унисекс Printio Totem cancer
                            530 Руб
                              Role of Proto-oncogenes in Bladder Cancer in Kashmiri Population sunishtha s yadav snps cancer incidences Role of Proto-oncogenes in Bladder Cancer in Kashmiri Population купить
                              Role of Proto-oncogenes in Bladder Cancer in Kashmiri Population
                              7790 Руб
                                Cancer a class of dreaded diseases - its challenges and remedies sunishtha s yadav snps cancer incidences Cancer a class of dreaded diseases - its challenges and remedies купить
                                Cancer a class of dreaded diseases - its challenges and remedies
                                4139 Руб
                                Massively parallel sequencing technology has a wide range of applications. Its use in SNP detection is already widespread and promises results of high accuracy. The aim is to validate data generated with the Genome Analyser II. SNPs, detected by using different mapping and SNP estimation parameters implemented in the bioinformatics tool CLC were compared to SNPs that are detected by using the method of conventional Sanger sequencing. Lines of D. mauritiana were used. As reference genome in CLC I was bound to use the genome of D. simulans, because there is no available genome of D. mauritiana until now. Using a stringent parameter set (short parameter set) that allows few mismatches of reads when mapping against the reference genome, only few regions are recovered which show divergence to the reference. 60%-81% SNPs detected with this parameter set are false positive ones compared to Sanger sequencing. If a less stringent parameter set is used this results in a very high number of false positive SNPs. Two times more SNPs were recovered with this parameter set, thereof 70-80% are false positive ones. Based on my results I drew conclude that the best approach for SNP detection is to make one first run permitting a high number of mismatches. The next step should be the use of more stringent values to reduce the high number of false positive SNPs.

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